Hplc (Reversed Phase Liquid Chromatography) coupled with MS (Mass Spectrometry), in this case tandem Mass Spectrometry or MS/MS can be used for rapid identification and quantitation of mycotoxins in food stuffs.

Mycotoxins can be separated by reversed-phase HPLC and detected using an electrospray ionization interface (ESI) and tandem MS, using MRM (multiple reaction monitoring) in the positive ion mode, The best approach is to have an all in one method that can detect all of the following compounds 4 main aflatoxins (B1,B2,G1, G2) and Ochratoxin A:

The goal is to find a fast method that would not require additional instrumentation such as an HPLC with Fluorescence Detector or ELISA since LCMSMS is used for many other assays including pesticides residue analysis. An example analysis is shown below:

Example TIC of MRM LCMSMS analysis of Mycotoxin Testing.